プレート正規化計算ツール

計算の考え方

Plate normalization is the dilution step that takes a counted cell suspension and brings it to a fixed target concentration (e.g. 100,000 cells/mL) before plating. At that working concentration, dispensing a fixed volume per well guarantees a fixed number of cells per well across the plate.

なぜ使うのか

Every cell-based assay is more reproducible when every well sees the same inoculum. Normalization separates the "how much of the cell stock do I dilute" question from the "how much do I plate" question, and makes the latter a single pipette volume.

この計算ツールが役立つ理由

Two concentrations and a target volume drive the answer, but the dead-volume excess needed to cover pipetting losses and edge effects is the part that turns a tiny math answer into a working bench volume. The calculator handles both, and surfaces the warnings that make a 96-well plate work first time.

主な前提条件

• The source concentration is a representative count of the well-mixed suspension.
• Mixing is uniform after the dilution — gentle inversion or trituration is enough.
• The number of wells is the actual wells you intend to fill (not the plate format).

制限事項

• It does not model cell settling during long pipetting sessions — resuspend periodically.
• It does not include media volume per well — the working suspension is what you dispense, not the final culture volume.
• It does not adjust for cells that change concentration after adherence or division.